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Optimization of an adenovirus-vectored zoster vaccine production process with chemically defined medium and a perfusion system
CONCLUSION: This optimization strategy could be used to develop a robust process with stable cell culture performance and adenovirus titer. Increasing PF-68 concentration in chemically defined medium could protect cells from shear stress generated by perfusion system.PMID:36183022 | DOI:10.1007/s10529-022-03302-6
Source: Biotechnology Letters - October 1, 2022 Category: Biotechnology Authors: Jianqi Nie Yang Sun He Ren Lingling Huang Kai Feng Ye Li Zhonghu Bai Source Type: research

Case 302: Supratentorial Lymphocytic Inflammation with Parenchymal Perivascular Enhancement Responsive to Steroids
Radiology. 2022 Jun;303(3):726-730. doi: 10.1148/radiol.204423.ABSTRACTPart one of this case appeared 4 months previously and may contain larger images. A 21-year-old immunocompetent man who was a long-term resident of Qatar presented to the emergency department with recurrent episodes of unprovoked generalized tonic-clonic seizures lasting 2-3 minutes that spontaneously resolved and were associated with postictal confusion. The patient also had progressive mild diplopia, intermittent dizziness, and numbness in the left arm over the course of 3 months. The patient did not have any other systemic symptoms or chronic medical...
Source: Herpes - May 23, 2022 Category: Infectious Diseases Authors: Surjith Vattoth Loai Aker Mohamed Abdelhady Ahmed H El Beltagi Source Type: research

Development of a perfusion process for serum-free adenovirus vector herpes zoster vaccine production
AMB Express. 2022 May 14;12(1):58. doi: 10.1186/s13568-022-01398-7.ABSTRACTHerpes zoster is caused by reactivation of the varicella zoster virus (VZV). Researching and developing a herpes zoster vaccine will help to decrease the incidence of herpes zoster. To increase the bioreactor productivity, a serum-free HEK293 cell perfusion process with adenovirus vector herpes zoster (rAd-HZ) vaccine production was developed efficiently using the design of experiment (DoE) method. First, serum-free media for HEK293 cells were screened in both batch and semi-perfusion culture modes. Then, three optimal media were employed in a mediu...
Source: Herpes - May 14, 2022 Category: Infectious Diseases Authors: Yang Sun Lingling Huang Jianqi Nie Kai Feng Yupeng Liu Zhonghu Bai Source Type: research

The efficient development of a novel recombinant adenovirus zoster vaccine perfusion production process
This study demonstrates the perfusion process to be superior strategy for adenovirus-based vaccine production compared to the batch-mode strategy (1,467 IFU/cell). Furthermore, our PATFP system shows potential to be comparable to the XCell ATF® system, and it would become an alternative perfusion strategy for the vaccine production.PMID:35216843 | PMC:PMC8863426 | DOI:10.1016/j.vaccine.2022.02.024
Source: Vaccine - February 26, 2022 Category: Allergy & Immunology Authors: Jianqi Nie Yang Sun Kai Feng Lingling Huang Ye Li Zhonghu Bai Source Type: research

Case 302
Radiology. 2022 Feb;302(2):481-483. doi: 10.1148/radiol.204422.ABSTRACTHistory A 21-year-old immunocompetent man who was a long-term resident of Qatar presented to the emergency department with recurrent episodes of unprovoked generalized tonic-clonic seizures lasting 2-3 minutes that spontaneously resolved and were associated with postictal confusion. The patient also had progressive mild diplopia, intermittent dizziness, and numbness in the left arm over the course of 3 months. The patient did not have any other systemic symptoms or chronic medical diseases. He did not have any history of intake of illicit drugs, supplem...
Source: Herpes - January 24, 2022 Category: Infectious Diseases Authors: Surjith Vattoth Loai Aker Mohamed Abdelhady Ahmed H El Beltagi Source Type: research